Key Research Articles
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Key Reviews
- The molecular machines that traffic signaling receptors into and out of cilia. Current Opinions in Cell Biology. 2018. Nachury MV
- How do cilia organize signaling cascades? Philosophical Transactions of the Royal Society. 2014. Nachury MV.
- Trafficking to the Ciliary Membrane: How to Get Across the Periciliary Diffusion Barrier? Annual Reviews of Cell and Developmental Biology. 2010. Nachury MV, Seeley ES and Jin H.
All Research Articles
2018
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Nature Genetics, 50:460–471. (posted 6/27/17 on bioRxiv 156059)
Breslow DK†*, Hoogendoorn S*, Kopp AR, Morgens DW, Vu BK, Kennedy MC, Han K, Li A, Hess GT, Bassik MC, Chen JK† and Nachury MV†. [† corresponding author][* equal contribution]
BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.
J Cell Biol, 217: 1847-1868. (posted 8/27/17 on bioRxiv 180620)
Ye F*, Nager AR* and Nachury MV. [* equal contribution]
Biosights: A 5min podcast by Ben Short from the JCB
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Nature Genetics, 50:460–471. (posted 6/27/17 on bioRxiv 156059)
Breslow DK†*, Hoogendoorn S*, Kopp AR, Morgens DW, Vu BK, Kennedy MC, Han K, Li A, Hess GT, Bassik MC, Chen JK† and Nachury MV†. [† corresponding author][* equal contribution]
BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.
J Cell Biol, 217: 1847-1868. (posted 8/27/17 on bioRxiv 180620)
Ye F*, Nager AR* and Nachury MV. [* equal contribution]
Biosights: A 5min podcast by Ben Short from the JCB
2017
BORC Regulates the Axonal Transport of Synaptic Vesicle Precursors by Activating ARL-8.
Current Biology. 27: 2569-2578.e4
Niwa S†*, Tao L*, Lu SY, Liew GM, Feng W, Nachury MV†, Shen K†. [† corresponding author][* equal contribution]
Microtubules acquire resistance from mechanical breakage through intralumenal acetylation.
Science. 356: 328–32
Xu Z, Schaedel L, Portran D, Marinkovich MP, Gaillard J, Théry M and Nachury MV
Tubulin acetylation protects long-lived microtubules against mechanical ageing
Nat Cell Biol. 19:391-398
Portran D, Schaedel L, Xu Z, Théry M, Nachury MV
An Actin Network Dispatches Ciliary GPCRs into Extracellular Vesicles to Modulate Signaling.
Cell. 168:252-263.e14
Nager AR, Goldstein JS, Herranz-Pérez V, Portran D, Ye F, Garcia-Verdugo JM and Nachury MV
BORC Regulates the Axonal Transport of Synaptic Vesicle Precursors by Activating ARL-8.
Current Biology. 27: 2569-2578.e4
Niwa S†*, Tao L*, Lu SY, Liew GM, Feng W, Nachury MV†, Shen K†. [† corresponding author][* equal contribution]
Microtubules acquire resistance from mechanical breakage through intralumenal acetylation.
Science. 356: 328–32
Xu Z, Schaedel L, Portran D, Marinkovich MP, Gaillard J, Théry M and Nachury MV
Tubulin acetylation protects long-lived microtubules against mechanical ageing
Nat Cell Biol. 19:391-398
Portran D, Schaedel L, Xu Z, Théry M, Nachury MV
An Actin Network Dispatches Ciliary GPCRs into Extracellular Vesicles to Modulate Signaling.
Cell. 168:252-263.e14
Nager AR, Goldstein JS, Herranz-Pérez V, Portran D, Ye F, Garcia-Verdugo JM and Nachury MV
2016
Cytoplasmic Dynein Antagonists with Improved Potency and Isoform Selectivity
ACS Chem Biol. 11:53-60
See SK, Hoogendoorn S, Chung AH, Ye F, Steinman JB, Sakata-Kato T, Miller RM, Cupido T, Zalyte R, Carter AP, Nachury MV, Kapoor TM, Chen JK
Loss of the BBSome perturbs endocytic trafficking and disrupts virulence of Trypanosoma brucei.
Proc Natl Acad Sci U S A. 113:632-7
Langousis G, Shimogawa MM, Saada EA, Vashisht AA, Spreafico R, Nager AR, Barshop WD, Nachury MV, Wohlschlegel JA, Hill KL.
Cytoplasmic Dynein Antagonists with Improved Potency and Isoform Selectivity
ACS Chem Biol. 11:53-60
See SK, Hoogendoorn S, Chung AH, Ye F, Steinman JB, Sakata-Kato T, Miller RM, Cupido T, Zalyte R, Carter AP, Nachury MV, Kapoor TM, Chen JK
Loss of the BBSome perturbs endocytic trafficking and disrupts virulence of Trypanosoma brucei.
Proc Natl Acad Sci U S A. 113:632-7
Langousis G, Shimogawa MM, Saada EA, Vashisht AA, Spreafico R, Nager AR, Barshop WD, Nachury MV, Wohlschlegel JA, Hill KL.
2015
Proteomics of Primary Cilia by Proximity Labeling
Developmental Cell. 35, 497–512
Mick DU, Rodrigues RB, Leib RD, Adams CM, Chien AS, Gygi SP and Nachury MV
Microtubules self-repair in response to mechanical stress.
Nat. Materials. 14:1156-63.
Schaedel L, Gaillard J, John K, Nachury MV, Blanchoin L and Théry M
Structural basis for Notch1 engagement of Delta-like 4
Science. 347:847-53.
Luca VC, Jude KM, Pierce NW, Nachury MV, Fischer S, Garcia KC.
Proteomics of Primary Cilia by Proximity Labeling
Developmental Cell. 35, 497–512
Mick DU, Rodrigues RB, Leib RD, Adams CM, Chien AS, Gygi SP and Nachury MV
Microtubules self-repair in response to mechanical stress.
Nat. Materials. 14:1156-63.
Schaedel L, Gaillard J, John K, Nachury MV, Blanchoin L and Théry M
Structural basis for Notch1 engagement of Delta-like 4
Science. 347:847-53.
Luca VC, Jude KM, Pierce NW, Nachury MV, Fischer S, Garcia KC.
2014
Structural basis for membrane targeting of the BBSome by ARL6
Nat Struct Mol Biol. 21: 1035-41
Mourão A, Nager AR, Nachury MV, Lorentzen E
The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3
Developmental Cell. 31: 265–278
Liew GM, Ye F, Nager AR, Murphy JP, Lee JS, Aguiar M, Breslow DK, Gygi SP and Nachury MV
The oral-facial-digital syndrome gene C2CD3 encodes a positive regulator of centriole elongation.
Nature Genetics. 46:905-911
Thauvin-Robinet C*, Lee JS*, Lopez E, Herranz-Pérez V, Shida T, Franco B, Jego L, Ye F, Pasquier L, Loget P, Gigot N, Aral B, Lopes CA, St-Onge J, Bruel AL, Thevenon J, González-Granero S, Alby C, Munnich A, Vekemans M, Huet F, Fry AM, Saunier S, Rivière JB, Attié-Bitach T, Garcia-Verdugo JM, Faivre L, Mégarbané A, Nachury MV. [* equal contribution]
α-Tubulin K40 Acetylation is Required for Contact Inhibition of Proliferation and Cell-Substrate Adhesion
Molecular Biology of the Cell. 25:257-266
Aguilar A, Becker L, Tedeschi T, Heller S, Iomini C, Nachury MV.
Exome sequencing of Bardet-Biedl syndrome patient identifies a null mutation in the BBSome subunit BBIP1 (BBS18)
J Med Genet. 51:132-126
Scheidecker S*, Etard C*, Pierce NW*, Geoffroy V, Schaefer E, Muller J, Chennen K, Flori E, Pelletier V, Poch O, Marion V, Stoetzel C, Strähle U, Nachury MV, Dollfus H. [* equal contribution]
Effects of tubulin acetylation and tubulin acetyltransferase binding on microtubule structure.
Mol Biol Cell. 25:257-266
Howes SC, Alushin GM, Shida T, Nachury MV, Nogales E.
2013
An in vitro assay for entry into cilia reveals unique properties of the soluble diffusion barrier.
J Cell Biol. 203:129-47.
Breslow DK, Koslover EF, Seydel F, Spakowitz AJ, Nachury MV.
αTAT1 catalyses microtubule acetylation at clathrin-coated pits.
Nature. 502:567-570
Montagnac G, Meas-Yedid V, Irondelle M, Castro-Castro A, Franco M, Shida T, Nachury MV, Benmerah A, Olivo-Marin JC, Chavrier P.
Single molecule imaging reveals a major role for diffusion in the exploration of ciliary space by signaling receptors.
Elife 2:e00654
Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ†, Nachury MV†. [† corresponding author]
2012
Structure of the α-tubulin acetyltransferase, αTAT1, and implications for tubulin-specific acetylation.
PNAS 109:19655-60.
Friedmann DR, Aguilar A, Fan J, Nachury MV, Marmorstein R.
2011
A Novel Protein LZTFL1 Regulates Ciliary Trafficking of the BBSome and Smoothened.
PLoS Genetics 7: e1002358.
Seo S, Zhang Q, Bugge K, Breslow DK, Searby CC, Nachury MV, Sheffield VC.
Primary cilia membrane assembly is initiated by Rab11- and TRAPPII-dependent trafficking of Rabin8 to the centrosome.
PNAS 108: 2759-64
Westlake CJ, Baye LM, Nachury MV, Wright KJ, Ervin KE, Phu L, Chalouni C, Beck JS, Kirkpatrick DS, Slusarski DC, Sheffield VC, Scheller RH and Jackson PK.
Structural basis for membrane targeting of the BBSome by ARL6
Nat Struct Mol Biol. 21: 1035-41
Mourão A, Nager AR, Nachury MV, Lorentzen E
The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3
Developmental Cell. 31: 265–278
Liew GM, Ye F, Nager AR, Murphy JP, Lee JS, Aguiar M, Breslow DK, Gygi SP and Nachury MV
The oral-facial-digital syndrome gene C2CD3 encodes a positive regulator of centriole elongation.
Nature Genetics. 46:905-911
Thauvin-Robinet C*, Lee JS*, Lopez E, Herranz-Pérez V, Shida T, Franco B, Jego L, Ye F, Pasquier L, Loget P, Gigot N, Aral B, Lopes CA, St-Onge J, Bruel AL, Thevenon J, González-Granero S, Alby C, Munnich A, Vekemans M, Huet F, Fry AM, Saunier S, Rivière JB, Attié-Bitach T, Garcia-Verdugo JM, Faivre L, Mégarbané A, Nachury MV. [* equal contribution]
α-Tubulin K40 Acetylation is Required for Contact Inhibition of Proliferation and Cell-Substrate Adhesion
Molecular Biology of the Cell. 25:257-266
Aguilar A, Becker L, Tedeschi T, Heller S, Iomini C, Nachury MV.
Exome sequencing of Bardet-Biedl syndrome patient identifies a null mutation in the BBSome subunit BBIP1 (BBS18)
J Med Genet. 51:132-126
Scheidecker S*, Etard C*, Pierce NW*, Geoffroy V, Schaefer E, Muller J, Chennen K, Flori E, Pelletier V, Poch O, Marion V, Stoetzel C, Strähle U, Nachury MV, Dollfus H. [* equal contribution]
Effects of tubulin acetylation and tubulin acetyltransferase binding on microtubule structure.
Mol Biol Cell. 25:257-266
Howes SC, Alushin GM, Shida T, Nachury MV, Nogales E.
2013
An in vitro assay for entry into cilia reveals unique properties of the soluble diffusion barrier.
J Cell Biol. 203:129-47.
Breslow DK, Koslover EF, Seydel F, Spakowitz AJ, Nachury MV.
αTAT1 catalyses microtubule acetylation at clathrin-coated pits.
Nature. 502:567-570
Montagnac G, Meas-Yedid V, Irondelle M, Castro-Castro A, Franco M, Shida T, Nachury MV, Benmerah A, Olivo-Marin JC, Chavrier P.
Single molecule imaging reveals a major role for diffusion in the exploration of ciliary space by signaling receptors.
Elife 2:e00654
Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ†, Nachury MV†. [† corresponding author]
2012
Structure of the α-tubulin acetyltransferase, αTAT1, and implications for tubulin-specific acetylation.
PNAS 109:19655-60.
Friedmann DR, Aguilar A, Fan J, Nachury MV, Marmorstein R.
2011
A Novel Protein LZTFL1 Regulates Ciliary Trafficking of the BBSome and Smoothened.
PLoS Genetics 7: e1002358.
Seo S, Zhang Q, Bugge K, Breslow DK, Searby CC, Nachury MV, Sheffield VC.
Primary cilia membrane assembly is initiated by Rab11- and TRAPPII-dependent trafficking of Rabin8 to the centrosome.
PNAS 108: 2759-64
Westlake CJ, Baye LM, Nachury MV, Wright KJ, Ervin KE, Phu L, Chalouni C, Beck JS, Kirkpatrick DS, Slusarski DC, Sheffield VC, Scheller RH and Jackson PK.
2010
The major α-tubulin K40 acetyltransferase αTAT1 promotes rapid ciliogenesis and efficient mechanosensation.
PNAS 107: 21517-22
Shida T, Cueva JG, Xu Z, Goodman MB† and Nachury MV†. [† corresponding author]
A septin diffusion barrier at the base of the primary cilium maintains ciliary membrane protein distribution.
Science. 329: 436-9
Hu Q, Milenkovic L, Jin H, Scott MP, Nachury MV, Spiliotis ET and Nelson WJ.
The conserved Bardet-Biedl Syndrome proteins assemble a coat that traffics membrane proteins to cilia.
Cell. 141: 1208-19.
Jin H, White SR, Shida T, Schulz S, Aguiar M, Gygi SP, Bazan JF, and Nachury MV
The major α-tubulin K40 acetyltransferase αTAT1 promotes rapid ciliogenesis and efficient mechanosensation.
PNAS 107: 21517-22
Shida T, Cueva JG, Xu Z, Goodman MB† and Nachury MV†. [† corresponding author]
A septin diffusion barrier at the base of the primary cilium maintains ciliary membrane protein distribution.
Science. 329: 436-9
Hu Q, Milenkovic L, Jin H, Scott MP, Nachury MV, Spiliotis ET and Nelson WJ.
The conserved Bardet-Biedl Syndrome proteins assemble a coat that traffics membrane proteins to cilia.
Cell. 141: 1208-19.
Jin H, White SR, Shida T, Schulz S, Aguiar M, Gygi SP, Bazan JF, and Nachury MV
2008
A Novel BBSome Subunit Regulates Microtubule Stability and Acetylation.
Developmental Cell. 15: 854–865..
Loktev AV, Zhang Q, Beck JS, Searby CC, Scheetz TE, Bazan JF, Slusarski DC, Sheffield VC, Jackson PK and Nachury MV
A Novel BBSome Subunit Regulates Microtubule Stability and Acetylation.
Developmental Cell. 15: 854–865..
Loktev AV, Zhang Q, Beck JS, Searby CC, Scheetz TE, Bazan JF, Slusarski DC, Sheffield VC, Jackson PK and Nachury MV
2007
A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis.
Cell. 129:1201-1213.
Nachury MV, Loktev AV, Zhang Q, Westlake CJ, Peränen J, Merdes A, Slusarski DC, Scheller RH, Bazan JF, Sheffield VC, and Jackson PK.
A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis.
Cell. 129:1201-1213.
Nachury MV, Loktev AV, Zhang Q, Westlake CJ, Peränen J, Merdes A, Slusarski DC, Scheller RH, Bazan JF, Sheffield VC, and Jackson PK.
All Reviews
The molecular machines that traffic signaling receptors into and out of cilia.
Current Opinions in Cell Biology. Cell Signaling. (2018) 51:124–131.
Nachury MV
How do cilia organize signaling cascades?
Philosophical Transactions of the Royal Society. (2014) 369: 20130465.
Nachury MV
Cilia Grow by Taking a Bite out of the Cell.
Developmental Cell. (2013) 27:126-7.
Pierce NW, Nachury MV
Primary cilia: how to keep the riff-raff in the plasma membrane.
Current Biology (2011) 11: R434-6.
Breslow DK and Nachury MV.
Trafficking to the Ciliary Membrane: How to Get Across the Periciliary Diffusion Barrier?
Annual Reviews of Cell and Developmental Biology (2010) 26: 59-87.
Nachury MV, Seeley ES and Jin H.
The perennial organelle: assembly and disassembly of the primary cilium.
Journal of Cell Science (2010) 123: 511-518.
Seeley ES and Nachury MV.
Constructing and Deconstructing Roles for the Primary Cilium in Tissue Architecture and Cancer.
Methods in Cell Biology (2009) 94: 299-313.
Seeley ES and Nachury MV.
The BBSome.
Current Biology (2009) 19: R472-3.
Jin H and Nachury MV.
Current Opinions in Cell Biology. Cell Signaling. (2018) 51:124–131.
Nachury MV
How do cilia organize signaling cascades?
Philosophical Transactions of the Royal Society. (2014) 369: 20130465.
Nachury MV
Cilia Grow by Taking a Bite out of the Cell.
Developmental Cell. (2013) 27:126-7.
Pierce NW, Nachury MV
Primary cilia: how to keep the riff-raff in the plasma membrane.
Current Biology (2011) 11: R434-6.
Breslow DK and Nachury MV.
Trafficking to the Ciliary Membrane: How to Get Across the Periciliary Diffusion Barrier?
Annual Reviews of Cell and Developmental Biology (2010) 26: 59-87.
Nachury MV, Seeley ES and Jin H.
The perennial organelle: assembly and disassembly of the primary cilium.
Journal of Cell Science (2010) 123: 511-518.
Seeley ES and Nachury MV.
Constructing and Deconstructing Roles for the Primary Cilium in Tissue Architecture and Cancer.
Methods in Cell Biology (2009) 94: 299-313.
Seeley ES and Nachury MV.
The BBSome.
Current Biology (2009) 19: R472-3.
Jin H and Nachury MV.
Methods
Analysis of soluble protein entry into primary cilia using semipermeabilized cells.
Methods Cell Biol. (2015) 127:203-21.
Breslow DK and Nachury MV
Tandem Affinity Purification of the BBSome, a Critical Regulator of Rab8 in Ciliogenesis.
Methods in Enzymology (2008) 439. 501-513.
Nachury MV.
Methods Cell Biol. (2015) 127:203-21.
Breslow DK and Nachury MV
Tandem Affinity Purification of the BBSome, a Critical Regulator of Rab8 in Ciliogenesis.
Methods in Enzymology (2008) 439. 501-513.
Nachury MV.
Essays
Give chance a chance
Molecular Biology of the Cell. (2011) 21:3919-20.
Nachury MV
Maxence Nachury: a transporting view of the primary cilium.
Journal of Cell Biology (2010) 191:436-7.
Interview by Ben Short.
Molecular Biology of the Cell. (2011) 21:3919-20.
Nachury MV
Maxence Nachury: a transporting view of the primary cilium.
Journal of Cell Biology (2010) 191:436-7.
Interview by Ben Short.
BC (Before Cilia)
A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
Cell (2005) 121:223-34.
Blower MD*, Nachury MV*, Heald R, and Weis K. [*equal contribution]
Importin β is a mitotic target of the small GTPase Ran in spindle assembly.
Cell (2001) 104: 95-106.
Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, Weis K.
The direction of transport through the nuclear pore can be inverted.
PNAS (1999) 96: 9622-9627.
Nachury MV, Weis K.
Cloning and characterization of hSRP1γ, a tissue-specific nuclear transport factor.
PNAS (1998) 95: 582-587.
Nachury MV, Ryder UW, Lamond AI, Weis K.
Cell (2005) 121:223-34.
Blower MD*, Nachury MV*, Heald R, and Weis K. [*equal contribution]
Importin β is a mitotic target of the small GTPase Ran in spindle assembly.
Cell (2001) 104: 95-106.
Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, Weis K.
The direction of transport through the nuclear pore can be inverted.
PNAS (1999) 96: 9622-9627.
Nachury MV, Weis K.
Cloning and characterization of hSRP1γ, a tissue-specific nuclear transport factor.
PNAS (1998) 95: 582-587.
Nachury MV, Ryder UW, Lamond AI, Weis K.